![](https://parts.igem.org/images/partbypart/icon_reporter.png)
Part:BBa_K1431813:Experience
Contents
Applications of BBa_K1431813
After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431813 and another part with promoter BBa_J23106, a relatively weak promoter.
LB agar plate growth comparison
We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.
JA is J23100,JB is J23106.
At 00:30 (16th,Oct.)
![K11-1.png](https://static.igem.org/mediawiki/parts/9/9b/K11-1.png)
At 1:50 (16th,Oct.)
![K11-2.png](https://static.igem.org/mediawiki/parts/2/22/K11-2.png)
At 3:00 (16th,Oct.)
![K11-3.png](https://static.igem.org/mediawiki/parts/8/84/K11-3.png)
At 6:00 (16th,Oct.)
![K11-4.png](https://static.igem.org/mediawiki/parts/f/f8/K11-4.png)
At 7:40 (16th,Oct.)
![K11-5.png](https://static.igem.org/mediawiki/parts/2/25/K11-5.png)
At 8:00 (16th,Oct.)
![K11-6.png](https://static.igem.org/mediawiki/parts/c/cb/K11-6.png)
At 10:00 (16th,Oct.)
![K11-7.png](https://static.igem.org/mediawiki/parts/d/dd/K11-7.png)
At 11:20 (16th,Oct.)
![K11-8.png](https://static.igem.org/mediawiki/parts/c/c4/K11-8.png)
LB broth growth comparison
We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol. Then we isolated single colonies from the plates. ===BBa_K1431813 didn't grow on the plate, so single colonies for comparison was isolated.===
At 00:50 (17th,Oct.)
![K11-1.jpg](https://static.igem.org/mediawiki/parts/5/52/K11-1.jpg)
At 02:45 (17th,Oct.)
![K11-2.jpg](https://static.igem.org/mediawiki/parts/f/f9/K11-2.jpg)
At 04:55 (17th,Oct.)
![K11-3.jpg](https://static.igem.org/mediawiki/parts/3/3f/K11-3.jpg)
At 10:40 (17th,Oct.)
![K11-4.jpg](https://static.igem.org/mediawiki/parts/4/4a/K11-4.jpg)
Conclusion
Unfortunately, BBa_K1431813 didn't grow and there isn't any color on plates or in broth!
User Reviews
UNIQ8a16ae6f0a6e3354-partinfo-00000000-QINU UNIQ8a16ae6f0a6e3354-partinfo-00000001-QINU